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4 February 2015
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QuantStudio
1
3D Digital PCR System
1
Contents
3
(Optional)
5
About this guide
8
Prerequisites
9
Instrument hardware
9
Instrument software
9
Instrument consumables
10
Getting Started
11
Data collection
12
Instrument filters
12
Operating the instrument
13
Prepare the QuantStudio
14
Plan the
15
Choose additional
16
Install the
17
Digital PCR
17
Instrument
17
IMPORTANT! The QuantStudio
20
The QuantStudio
21
Install the thermal
23
IMPORTANT! The ProFlex
24
PCR System
26
CAUTION HOT
27
Install the GeneAmp
29
PCR System 9700
29
IMPORTANT! The GeneAmp
30
IMPORTANT! If the GeneAmp
31
Connect the QuantStudio
34
3D Instrument to a network
34
3D AnalysisSuite
35
Software
35
Prepare Samples and Load
36
Reactions
36
Sample dilution
37
Determine the
37
Prepare the
39
X0300036
41
Load and seal the
43
IMPORTANT! Press the
45
Load chips manually
50
Digital PCR 20K
51
IMPORTANT! Allow the
53
Perform the PCR
58
08:00 AM 4/25/13 25.0°C
60
GeneAmp® PCR System 9700
60
Run UserUtilEditCreate
60
Version: 3.12
60
Materials
61
Guidelines for
62
Unload the
64
Analyze the Prepared Chips
66
Analysis workflow
67
About the
68
Analyze the chips
69
Analyzing the data
73
About the results
74
About data quality
75
About the data
75
Transfer the
76
Using the QuantStudio
77
Troubleshooting
79
(QuantStudio
82
IMPORTANT! Condensation
83
Maintenance
86
Clean the chip tray
87
Returning your QuantStudio
89
3D Instrument for service
89
Replace the instrument fuses
90
Replace the fuses
91
Update the
92
Calibrate the touchscreen
93
View the instrument log
93
Networking
95
Network setup
96
Required network
97
Connect the
98
Instrument to a
98
Create a network
103
Parts and Materials
106
Specifications and Layout
109
Instrument clearances
110
Electrical specifications
111
Environmental requirements
112
Network requirements
113
Symbols on this instrument
114
Conformity
115
Location of safety
116
Fisher Scientific
117
Instrument safety
117
3D Digital PCR Chip Loader
118
Chemical safety
119
Biological hazard safety
120
Documentation and support
121
4 February 2015
122
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